Andrew Gardner, Ph.D.

Chief Scientific Officer

With nearly three decades of research experience, Andy has been a leader in investigating how extremophilic organisms living in high temperature environments copy and repair their genome. Much of his research program at New England Biolabs, aimed to understand the biochemistry of individual replication and repair enzymes and how they work together to accomplish fast and accurate synthesis. His expertise extends to developing new innovative enzyme-based molecular workflows and technologies, resulting in many publications, invited presentations, patents and successful commercial products. In addition to running a research lab, Andy was the Scientific Director of the Molecular Enzymology Research Division at New England Biolabs, where he led world-class scientific teams focused on enzyme discovery, characterization and technology development for molecular diagnostics, next-generation sequencing, and synthetic biology. Andy also enjoys mentoring students and Post-Doctoral Fellows in the lab as well as teaching at Endicott College, Tufts University and at the New England Biolabs Molecular Biology Summer Workshop. Andy earned a Ph.D. in Cell and Molecular Biology from Boston University, an A.L.M. in Biology from Harvard University, and a B.A. in Molecular Biology and Biochemistry from Middlebury College.

Education

1995 – BA Molecular Biology & Biochemistry, Middlebury College, Middlebury, VT
2002 – ALM Biology, Harvard University, Cambridge, MA
2010 – PhD Cell & Molecular Biology, Boston University, Boston, MA

Research Profile Links

LinkedIn Profile – Andy Gardner, Ph.D.

Google Scholar

Research Interests

My research interests focus on discovering and understanding enzymes from organisms that live in extreme environments such as marine hydrothermal vents. Remarkably, organisms not only survive but also thrive in these extreme environments where temperatures reach over 100°C. DNA sequencing projects at GMGI and others have highlighted the vast diversity of proteins from high temperature environments with little information about their function. Given the huge diversity of proteins and enzymes, my research will focus on developing tools to better understand enzyme function and their roles in key cellular processes required for life at high temperature.

Selected Publications

*for full list, see Google Scholar

Barton E. Slatko, Andrew F. Gardner and Frederick M. Ausubel (2018) Overview of Next-Generation Sequencing Technologies. Curr. Protoc. Mol. Biol. Apr;122(1):e59. doi: 10.1002/cpmb.59.

Kelly M. Zatopek, Vladimir Potapov, Lisa L. Maduzia, Ece Alpaslan, Lixin Chen, Thomas C. Evans, Jr., Jennifer L. Ong, Laurence M. Ettwiller and Andrew F. Gardner. (2019) RADAR-seq: A RAre DAmage and Repair sequencing method for detecting DNA damage on a genome-wide scale. DNA Repair (Amst). 80:36-44. doi: 10.1016/j.dnarep.2019.06.007

Kelly M. Zatopek*, Ece Alpaslan*, Thomas C Evans, Jr., Ludovic Sauguet and Andrew F. Gardner. (2020) Novel ribonucleotide discrimination in the RNA polymerase-like two-barrel catalytic core of Family D DNA polymerases. Nucleic Acids Research. 48(21):12204-12218.

Zatopek, K.M., Fossa, S.L., Bilotti, K., Caffrey, P.J., Chuzel, L., Gehring, A.M., Lohman, G.J.S., Taron, C.H., Gardner, A.F. (2021) Capillary electrophoresis-based functional genomics screening to discover novel archaeal DNA modifying enzymes. Appl. Environ. Micro. 88(2):e0213721.

Leonardo Betancurt-Anzola, Markel Martínez-Carranza, Marc Delarue, Kelly M. Zatopek, Andrew F. Gardner, Ludovic Sauguet. (2023) Molecular basis for proofreading by the unique exonuclease domain of Family-D DNA polymerases. Nat. Comm. Dec 14;14(1):8306.