Antibody Builders: Essentials of Mammalian Cell Culture & Biomanufacturing

Course Description

Massachusetts continues to be recognized as the world’s premier hub for the life sciences and biotechnology. In response to the ongoing need for entry-level talent within the industry, Gloucester Biotechnology Academy has developed an intensive, hands-on course designed to enhance skills crucial for careers in cell culture and biomanufacturing.

The curriculum features practical training on state-of-the-art instrumentation, allowing students to gain insight into how mammalian cell culture is used to develop small molecule therapies and scale their production in a manufacturing environment.

Training consists of 40 hours of laboratory experiments over a two-week session. In the first week, students will learn basic mammalian cell culture techniques. In week two, students will apply the mammalian cell culture techniques to create a monoclonal antibody in a bioreactor.

To accommodate working professionals, courses will be offered twice a day: in the morning (8:30a to 12:30p) and in the afternoon (1:00p to 5:00p). The curriculum in the morning and afternoon classes will be the same.

Please note, students enrolling in this program MUST be proficient in micropipetting, lab math, and solution prep.

Summer 2024 Information

Session Dates

Session 1: July 8 – July 19

Session 2: July 22 – August 2


55 Blackburn Center, Gloucester MA 01930

Registration Information

Learning Outcomes

Students who complete this course will be proficient in the following skills:

  • Following and maintaining detailed batch records
  • Working aseptically in a biosafety cabinet
  • Thawing, passaging, feeding, counting, and banking
  • Cryopreservation
  • Cell staining and microscopy
  • Preparing growth media and buffers
  • Assembling and maintaining a bioreactor
  • Transfecting mammalian cells
  • Developing a seed culture to inoculate a bioreactor
  • Monitoring cellular viability and predicting harvest times
  • Harvesting with centrifugation and filtration
  • Purifying antibodies with column chromatography
  • Evaluating antibody purity and yield with cellular assays

Featured Instrumentation

  • Biosafety cabinets
  • Manual and digital cell counters
  • Microscopes (inverted, compound light, fluorescent)
  • Bioreactors
  • Single-use vessels
  • AKTA Pure column chromatography
  • UNICORN software
  • High pressure liquid chromatography

Industry Roles

Students who complete this training will be qualified for the following roles:

  • Biomanufacturing Associate
  • Biomanufacturing Technician – Upstream/Downstream
  • Biomanufacturing Technician – QA/QC
  • Cell Culture Technician
  • Media Technician
  • Research Associate – Cell Culture/Cell Biology