When standard sequencing workflows don’t work
A GMGI eDNA Service Case Study
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The Challenge
Environmental samples don’t always behave like textbook samples — and sometimes getting an answer requires more than putting DNA through a standard sequencing pipeline.
A research team studying marine bacterial communities in the Caribbean encountered exactly that problem. Their environmental samples contained compounds that interfered with downstream molecular processing, and a previous service provider had been unable to successfully complete the sequencing workflow.
They brought the challenge to GMGI.
The GMGI Approach
Rather than stopping when the standard protocol failed, GMGI scientists treated the project as an R&D problem. Through systematic troubleshooting, method optimization, and careful quality control, the team identified a workable approach and successfully generated the sequencing data the researchers needed.
The Impact
Challenging environmental samples frequently contain inhibitors, low concentrations of target DNA, complex microbial communities, or other characteristics that require customized methods.
GMGI combines advanced sequencing infrastructure with hands-on molecular biology expertise. That means our team can troubleshoot difficult samples, adapt protocols, and develop solutions when an off-the-shelf workflow isn’t enough.
Our goal is not simply to generate sequences. It is to help turn environmental DNA into useful information.